SeedGenes Profile: EMB 2473

Gene Information Gene Symbol EMB 2473 Chromosome Locus At5g27540   Alleles in Database 1 Identity Confidence Confirmed Alias Symbol MIRO 1 (C. Leaver) Alternate Name Gene Class Embryo Defective Predicted Function Mitochondrial GTPase Function Details Regulation of mitochondrial morphology Gene Evidence Molecular Complementation C. Leaver (1 allele) Double Border Recovery Meinke / Syngenta (1 allele) Database Release March, 2003 Predicted Sequences Gene: 5030 nucleotides mRNA: 2452 nucleotides Protein:   648 amino acids cDNA Status FLcDNA Available: AY081280 Top BLAST Hits Arabidopsis: AT3G63150.1 (58%) Plant ESTs: Helianthus annuus (71%) Model Genomes:   Homo sapiens (36%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 246775_at

Mutant Information Allele Symbol emb 2473 Mutant Class Embryo Defective Source of Mutant Meinke / Syngenta Mutant Line Number 80307 Ecotype Columbia Genetic Background qrt ABRC Stock CS16182 Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence Details  (Help) Location of Mutation Intron 12 to Exon 13 Terminal Phenotype Preglobular  Details Nomarski Phenotype   Allelism Evidence Reference Allele Database Release March, 2003 Seed Color White Embryo Color White Percent Mutant Seeds 14.3 % Chi - Square 59.7 Seeds Counted 984 Percent Top Half 78.7 % Chi - Square 46.6 Average Seed Length 430 ± 30 µm Average Embryo Length 20 ± 40 µm