SeedGenes Profile: EMB 2474

Gene Information Gene Symbol EMB 2474 Chromosome Locus At3g46560   Alleles in Database 1 Identity Confidence Not Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Small Zinc Finger Protein Function Details Similar to mitochondrial inner membrane translocase (TIM9) Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Database Release September, 2002 Predicted Sequences Gene: 926 nucleotides mRNA: 538 nucleotides Protein:   93 amino acids cDNA Status FLcDNA Available: AY045940 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (95%) Model Genomes:   None Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 252479_at

Mutant Information Allele Symbol emb 2474 Mutant Class Embryo Defective Source of Mutant Meinke / Syngenta Mutant Line Number 68181 Ecotype Columbia Genetic Background qrt ABRC Stock CS16114 Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence Details  (Help) Location of Mutation Exon 2 Terminal Phenotype Preglobular  Details Nomarski Phenotype Early Globular  Images Allelism Evidence Reference Allele Database Release September, 2002 Seed Color White Embryo Color White Percent Mutant Seeds 22.3 % Chi - Square 4.1 Seeds Counted 1041 Percent Top Half 48.3 % Chi - Square 0.3 Average Seed Length 320 ± 80 µm Average Embryo Length 15 ± 0 µm