SeedGenes Profile: EMB 2729

Gene Information Gene Symbol EMB 2729 Chromosome Locus At3g20440   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function 1,4-alpha-glucan branching enzyme Function Details Similar to starch branching enzyme Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Border Recovery Meinke / Salk (1 allele) Database Release September, 2002 Predicted Sequences Gene: 7240 nucleotides mRNA: 3088 nucleotides Protein:   869 amino acids cDNA Status FLcDNA Available: AK118785 Top BLAST Hits Arabidopsis: AT2G36390.1 (39%) Plant ESTs: Rice (Oryza sativa) (63%) Model Genomes:   Homo sapiens (40%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 257667_at

Mutant Information Allele Symbol emb 2729-2 Mutant Class Embryo Defective Source of Mutant Meinke / Salk Mutant Line Number SALK_129083 Ecotype Columbia Genetic Background   ABRC Stock   Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence   Location of Mutation Intron 15 Terminal Phenotype Transition  Details Nomarski Phenotype   Allelism Evidence Complementation Test; Sequence Identity Database Release October, 2007 Seed Color White Embryo Color White Percent Mutant Seeds 24.4 % Chi - Square 0.1 Seeds Counted 283 Percent Top Half 47.8 % Chi - Square 0.1 Average Seed Length 540 ± 30 µm Average Embryo Length 130 ± 50 µm