SeedGenes Profile: EMB 2729

Gene Information Gene Symbol EMB 2729 Chromosome Locus At3g20440   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function 1,4-alpha-glucan branching enzyme Function Details Similar to starch branching enzyme Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Border Recovery Meinke / Salk (1 allele) Database Release September, 2002 Predicted Sequences Gene: 7240 nucleotides mRNA: 3088 nucleotides Protein:   869 amino acids cDNA Status FLcDNA Available: AK118785 Top BLAST Hits Arabidopsis: AT2G36390.1 (39%) Plant ESTs: Rice (Oryza sativa) (63%) Model Genomes:   Homo sapiens (40%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 257667_at

Mutant Information Allele Symbol emb 2729-1 emb 2729-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Salk Mutant Line Number 48882 SALK_129083 Ecotype Columbia Columbia Genetic Background     ABRC Stock CS16113   Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help)   Location of Mutation Intron 21 Intron 15 Terminal Phenotype Cotyledon  Details Transition  Details Nomarski Phenotype   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release September, 2002 October, 2007 Seed Color White White Embryo Color White White Percent Mutant Seeds 27.4 % 24.4 % Chi - Square 3.3 0.1 Seeds Counted 1085 283 Percent Top Half 47.8 % 47.8 % Chi - Square 0.6 0.1 Average Seed Length 530 ± 50 µm 540 ± 30 µm Average Embryo Length 230 ± 90 µm 130 ± 50 µm