SeedGenes Profile: EMB 2730

Gene Information Gene Symbol EMB 2730 Chromosome Locus At5g02250   Alleles in Database 5   See List OR Show All Identity Confidence Confirmed Alias Symbol RNR 1 (D. Stern) Sequence Identity RNR 1 (D. Stern) Complemetation Test Gene Class Embryo Defective Predicted Function Ribonuclease II Family Protein Function Details 3' to 5' exoribonuclease; Maturation of chloroplast rRNAs Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Border Recovery Meinke / Salk (1 allele) Border Recovery Stern / Salk (2 alleles) Border Recovery Meinke; Stern / Salk (1 allele) Database Release September, 2002 Predicted Sequences Gene: 4745 nucleotides mRNA: 2911 nucleotides Protein:   803 amino acids cDNA Status FLcDNA Available: AK176383 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Rice (Oryza sativa) (58%) Model Genomes:   Synechocystis (37%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 251040_at

Mutant Information Allele Symbol rnr 1-3 Mutant Class Seed Pigment Source of Mutant Meinke; Stern / Salk Mutant Line Number SALK_090294 Ecotype Columbia Genetic Background   ABRC Stock   Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence   Location of Mutation Exon 14 Terminal Phenotype Pigment Defective  Details Nomarski Phenotype   Allelism Evidence Complementation Test; Sequence Identity Database Release October, 2007 Seed Color Pale Green Embryo Color Pale Green Percent Mutant Seeds 26.2 % Chi - Square 0.2 Seeds Counted 259 Percent Top Half 44.1 % Chi - Square 0.9 Average Seed Length 550 ± 0 µm Average Embryo Length 450 ± 0 µm