OSU Syngenta vbi

SeedGenes Profile: EMB 2730

Gene Information
Gene Symbol EMB 2730
Chromosome Locus At5g02250   tair
Alleles in Database 5   See List OR Show All
Identity Confidence Confirmed
Alias Symbol RNR 1 (D. Stern) Sequence Identity
RNR 1 (D. Stern) Complemetation Test
Gene Class Embryo Defective
Predicted Function Ribonuclease II Family Protein
Function Details 3' to 5' exoribonuclease; Maturation of chloroplast rRNAs
Gene Evidence
Double Border Recovery
Meinke / Syngenta (1 allele)
Border Recovery
Meinke / Salk (1 allele)
Border Recovery
Stern / Salk (2 alleles)
Border Recovery
Meinke; Stern / Salk (1 allele)
Database Release September, 2002
Predicted Sequences
Gene:4745 nucleotides
mRNA: 2911 nucleotides
Protein:  803 amino acids
cDNA Status FLcDNA Available: AK176383
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Rice (Oryza sativa) (58%)
Model Genomes:  Synechocystis (37%)
Subcellular Localization TargetP Targeted to Chloroplasts
mRNA Expression Data NASCArray Spot History for 251040_at
Mutant Information
Allele Symbol rnr 1-3
Mutant Class Seed Pigment
Source of Mutant Meinke; Stern / Salk
Mutant Line Number SALK_090294
Ecotype Columbia
Genetic Background  
ABRC Stock  
Mutagen Treatment T-DNA
Insertion Mutant Yes   Details
Flanking Sequence  
Location of Mutation Exon 14
Terminal Phenotype Pigment Defective  Details
Nomarski Phenotype  
Allelism Evidence Complementation Test; Sequence Identity
Database Release October, 2007
Seed Color Pale Green
Embryo Color Pale Green
Percent Mutant Seeds 26.2 %
Chi - Square 0.2
Seeds Counted 259
Percent Top Half 44.1 %
Chi - Square 0.9
Average Seed Length 550 ± 0 µm
Average Embryo Length 450 ± 0 µm