SeedGenes Profile: EMB 2730

Gene Information Gene Symbol EMB 2730 Chromosome Locus At5g02250   Alleles in Database 5   See List OR Show All Identity Confidence Confirmed Alias Symbol RNR 1 (D. Stern) Sequence Identity RNR 1 (D. Stern) Complemetation Test Gene Class Embryo Defective Predicted Function Ribonuclease II Family Protein Function Details 3' to 5' exoribonuclease; Maturation of chloroplast rRNAs Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Border Recovery Meinke / Salk (1 allele) Border Recovery Stern / Salk (2 alleles) Border Recovery Meinke; Stern / Salk (1 allele) Database Release September, 2002 Predicted Sequences Gene: 4745 nucleotides mRNA: 2911 nucleotides Protein:   803 amino acids cDNA Status FLcDNA Available: AK176383 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Rice (Oryza sativa) (58%) Model Genomes:   Synechocystis (37%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 251040_at

Mutant Information Allele Symbol emb 2730-1 emb 2730-2 rnr 1-1 rnr 1-2 rnr 1-3 Mutant Class Embryo Defective Seed Pigment Seed Pigment Seed Pigment Seed Pigment Source of Mutant Meinke / Syngenta Meinke / Salk Stern / Salk Stern / Salk Meinke; Stern / Salk Mutant Line Number 11129 SALK_039364 SALK_138535 SALK_044726 SALK_090294 Ecotype Columbia Columbia Columbia Columbia Columbia Genetic Background           ABRC Stock CS16126         Mutagen Treatment T-DNA T-DNA T-DNA T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Yes   Details Yes   Details Yes   Details Flanking Sequence Details  (Help)         Location of Mutation Exon 14 to 3' UTR Exon 7 Exon 8 Intron 12 Exon 14 Terminal Phenotype Cotyledon  Details Pigment Defective  Details Pigment Defective Pigment Defective Pigment Defective  Details Nomarski Phenotype   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Sequence Identity Sequence Identity Complementation Test; Sequence Identity Database Release September, 2002 October, 2007 October, 2007 October, 2007 October, 2007 Seed Color White to Pale Yellow Pale Yellow     Pale Green Embryo Color White to Pale Yellow Pale Yellow     Pale Green Percent Mutant Seeds 24.0 % 23.7 %     26.2 % Chi - Square 0.6 0.3     0.2 Seeds Counted 1079 295     259 Percent Top Half 53.6 % 54.3 %     44.1 % Chi - Square 1.4 0.5     0.9 Average Seed Length 500 ± 40 µm 550 ± 0 µm     550 ± 0 µm Average Embryo Length 380 ± 60 µm 450 ± 0 µm     450 ± 0 µm