SeedGenes Profile: EMB 2733

Gene Information Gene Symbol EMB 2733 Chromosome Locus At1g32490   Alleles in Database 1 Identity Confidence Not Confirmed Alias Symbol ESP 3 (D. Baulcombe) Sequence Identity Gene Class Embryo Defective Predicted Function RNA Helicase (Prp2) Function Details Proposed role in pre-mRNA splicing Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Database Release March, 2003 Predicted Sequences Gene: 7653 nucleotides mRNA: 3583 nucleotides Protein:   1044 amino acids cDNA Status FLcDNA Available: AY074318 Top BLAST Hits Arabidopsis: AT2G35340.1 (82%) Plant ESTs: Rice (Oryza sativa) (69%) Model Genomes:   Danio rerio (53%) Subcellular Localization TargetP Targeted to Secretory Pathway SUBA mRNA Expression Data NASCArray Spot History for 260695_at

Mutant Information Allele Symbol emb 2733 Mutant Class Embryo Defective Source of Mutant Meinke / Syngenta Mutant Line Number 103268 Ecotype Columbia Genetic Background   ABRC Stock CS16184 Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence Details  (Help) Location of Mutation Intron 8 Terminal Phenotype Globular  Details Nomarski Phenotype   Images Allelism Evidence Reference Allele Database Release March, 2003 Seed Color Pale Yellow Embryo Color Pale Yellow Percent Mutant Seeds 25.4 % Chi - Square 0.1 Seeds Counted 1262 Percent Top Half 52.0 % Chi - Square 0.5 Average Seed Length 510 ± 40 µm Average Embryo Length 70 ± 20 µm