SeedGenes Profile: EMB 2734

Gene Information Gene Symbol EMB 2734 Chromosome Locus At5g19820   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Karyopherin Beta 3 Transportin Function Details Nuclear import of ribosomal proteins Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Single Border Recovery Meinke / Salk (1 allele) Database Release March, 2003 Predicted Sequences Gene: 6088 nucleotides mRNA: 3922 nucleotides Protein:   1116 amino acids cDNA Status FLcDNA Available: AY035044 Top BLAST Hits Arabidopsis: AT4G27640.1 (25%) Plant ESTs: Medicago truncatula (81%) Model Genomes:   Homo sapiens (37%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 245941_at

Mutant Information Allele Symbol emb 2734-2 Mutant Class Embryo Defective Source of Mutant Meinke / Salk Mutant Line Number SALK_050129 Ecotype Columbia Genetic Background   ABRC Stock CS24088 Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence   Location of Mutation Intron 10 Terminal Phenotype Cotyledon  Details Nomarski Phenotype   Images Allelism Evidence Complementation Test; Sequence Identity Database Release March, 2004 Seed Color Pale Green Embryo Color Pale Green Percent Mutant Seeds 28.6 % Chi - Square 3.5 Seeds Counted 511 Percent Top Half 53.4 % Chi - Square 0.6 Average Seed Length 520 ± 60 µm Average Embryo Length 370 ± 110 µm