SeedGenes Profile: EMB 2734

Gene Information Gene Symbol EMB 2734 Chromosome Locus At5g19820   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Karyopherin Beta 3 Transportin Function Details Nuclear import of ribosomal proteins Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Single Border Recovery Meinke / Salk (1 allele) Database Release March, 2003 Predicted Sequences Gene: 6088 nucleotides mRNA: 3922 nucleotides Protein:   1116 amino acids cDNA Status FLcDNA Available: AY035044 Top BLAST Hits Arabidopsis: AT4G27640.1 (25%) Plant ESTs: Medicago truncatula (81%) Model Genomes:   Homo sapiens (37%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 245941_at

Mutant Information Allele Symbol emb 2734-1 emb 2734-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Salk Mutant Line Number 104260 SALK_050129 Ecotype Columbia Columbia Genetic Background     ABRC Stock CS16185 CS24088 Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help)   Location of Mutation Intron 5 to Exon 6 Intron 10 Terminal Phenotype Cotyledon  Details Cotyledon  Details Nomarski Phenotype   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release March, 2003 March, 2004 Seed Color Pale Yellow-Green Pale Green Embryo Color Pale Yellow-Green Pale Green Percent Mutant Seeds 23.2 % 28.6 % Chi - Square 2.0 3.5 Seeds Counted 1168 511 Percent Top Half 53.5 % 53.4 % Chi - Square 1.3 0.6 Average Seed Length 510 ± 40 µm 520 ± 60 µm Average Embryo Length 270 ± 120 µm 370 ± 110 µm