SeedGenes Profile: EMB 2738

Gene Information Gene Symbol EMB 2738 Chromosome Locus At3g12080   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Uncertain Function Details Putative ribosome biogenesis GTPase (EngA) Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Single Border Recovery Meinke / Salk (1 allele) Database Release March, 2003 Predicted Sequences Gene: 4347 nucleotides mRNA: 2209 nucleotides Protein:   663 amino acids cDNA Status FLcDNA Available: AY080655 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Spruce (69%) Model Genomes:   Synechocystis (46%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 256274_at

Mutant Information Allele Symbol emb 2738-2 Mutant Class Embryo Defective Source of Mutant Meinke / Salk Mutant Line Number SALK_104755 Ecotype Columbia Genetic Background   ABRC Stock CS24090 Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence   Location of Mutation Intron 6 Terminal Phenotype Globular  Details Nomarski Phenotype   Images Allelism Evidence Complementation Test; Sequence Identity Database Release March, 2004 Seed Color White Embryo Color White Percent Mutant Seeds 25.2 % Chi - Square <0.1 Seeds Counted 1121 Percent Top Half 49.1 % Chi - Square <0.1 Average Seed Length 500 ± 60 µm Average Embryo Length 70 ± 30 µm