OSU Syngenta vbi

SeedGenes Profile: EMB 2738

Gene Information
Gene Symbol EMB 2738
Chromosome Locus At3g12080   tair
Alleles in Database 2   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function Uncertain
Function Details Putative ribosome biogenesis GTPase (EngA)
Gene Evidence
Double Border Recovery
Meinke / Syngenta (1 allele)
Single Border Recovery
Meinke / Salk (1 allele)
Database Release March, 2003
Predicted Sequences
Gene:4347 nucleotides
mRNA: 2209 nucleotides
Protein:  663 amino acids
cDNA Status FLcDNA Available: AY080655
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Spruce (69%)
Model Genomes:  Synechocystis (46%)
Subcellular Localization TargetP Targeted to Chloroplasts
SUBA
mRNA Expression Data NASCArray Spot History for 256274_at
    
Mutant Information
Allele Symbol emb 2738-1emb 2738-2
Mutant Class Embryo DefectiveEmbryo Defective
Source of Mutant Meinke / SyngentaMeinke / Salk
Mutant Line Number 121994SALK_104755
Ecotype ColumbiaColumbia
Genetic Background   
ABRC Stock CS16188CS24090
Mutagen Treatment T-DNAT-DNA
Insertion Mutant Yes   DetailsYes   Details
Flanking Sequence Details  (Help) 
Location of Mutation UpstreamIntron 6
Terminal Phenotype Globular  DetailsGlobular  Details
Nomarski Phenotype   Images  Images
Allelism Evidence Reference AlleleComplementation Test; Sequence Identity
Database Release March, 2003March, 2004
Seed Color WhiteWhite
Embryo Color WhiteWhite
Percent Mutant Seeds 24.7 %25.2 %
Chi - Square <0.1<0.1
Seeds Counted 10621121
Percent Top Half 48.8 %49.1 %
Chi - Square 0.1<0.1
Average Seed Length 520 ± 30 µm500 ± 60 µm
Average Embryo Length 100 ± 30 µm70 ± 30 µm