OSU Syngenta vbi

SeedGenes Profile: EMB 2746

Gene Information
Gene Symbol EMB 2746
Chromosome Locus At5g63420   tair
Alleles in Database 2   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function Metallo-Beta-Lactamase Family Protein
Function Details Putative chloroplast RNA endounclease (Rnase J)
Gene Evidence
Double Border Recovery
Meinke / Syngenta (1 allele)
Single Border Recovery
Meinke / Salk (1 allele)
Database Release March, 2003
Predicted Sequences
Gene:5583 nucleotides
mRNA: 3026 nucleotides
Protein:  911 amino acids
cDNA Status FLcDNA Available: AK221272
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Vitis vinifera (70%)
Model Genomes:  Synechocystis (33%)
Subcellular Localization TargetP Targeted to Chloroplasts
mRNA Expression Data NASCArray Spot History for 247385_at
Mutant Information
Allele Symbol emb 2746-2
Mutant Class Embryo Defective
Source of Mutant Meinke / Salk
Mutant Line Number SALK_110582
Ecotype Columbia
Genetic Background  
ABRC Stock CS24091
Mutagen Treatment T-DNA
Insertion Mutant Yes   Details
Flanking Sequence  
Location of Mutation Intron 7
Terminal Phenotype Globular  Details
Nomarski Phenotype   Images
Allelism Evidence Complementation Test; Sequence Identity
Database Release March, 2004
Seed Color White
Embryo Color White
Percent Mutant Seeds 24.0 %
Chi - Square 0.5
Seeds Counted 1124
Percent Top Half 49.6 %
Chi - Square <0.1
Average Seed Length 500 ± 60 µm
Average Embryo Length 70 ± 30 µm