SeedGenes Profile: EMB 2746

Gene Information Gene Symbol EMB 2746 Chromosome Locus At5g63420   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Metallo-Beta-Lactamase Family Protein Function Details Putative chloroplast RNA endounclease (Rnase J) Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Single Border Recovery Meinke / Salk (1 allele) Database Release March, 2003 Predicted Sequences Gene: 5583 nucleotides mRNA: 3026 nucleotides Protein:   911 amino acids cDNA Status FLcDNA Available: AK221272 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Vitis vinifera (70%) Model Genomes:   Synechocystis (33%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 247385_at

Mutant Information Allele Symbol emb 2746-2 Mutant Class Embryo Defective Source of Mutant Meinke / Salk Mutant Line Number SALK_110582 Ecotype Columbia Genetic Background   ABRC Stock CS24091 Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence   Location of Mutation Intron 7 Terminal Phenotype Globular  Details Nomarski Phenotype   Images Allelism Evidence Complementation Test; Sequence Identity Database Release March, 2004 Seed Color White Embryo Color White Percent Mutant Seeds 24.0 % Chi - Square 0.5 Seeds Counted 1124 Percent Top Half 49.6 % Chi - Square <0.1 Average Seed Length 500 ± 60 µm Average Embryo Length 70 ± 30 µm