SeedGenes Profile: EMB 2746

Gene Information Gene Symbol EMB 2746 Chromosome Locus At5g63420   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Metallo-Beta-Lactamase Family Protein Function Details Putative chloroplast RNA endounclease (Rnase J) Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Single Border Recovery Meinke / Salk (1 allele) Database Release March, 2003 Predicted Sequences Gene: 5583 nucleotides mRNA: 3026 nucleotides Protein:   911 amino acids cDNA Status FLcDNA Available: AK221272 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Vitis vinifera (70%) Model Genomes:   Synechocystis (33%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 247385_at

Mutant Information Allele Symbol emb 2746-1 emb 2746-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Salk Mutant Line Number 128084 SALK_110582 Ecotype Columbia Columbia Genetic Background     ABRC Stock CS16191 CS24091 Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help)   Location of Mutation Exon 9 Intron 7 Terminal Phenotype Globular  Details Globular  Details Nomarski Phenotype   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release March, 2003 March, 2004 Seed Color White White Embryo Color White White Percent Mutant Seeds 25.1 % 24.0 % Chi - Square <0.1 0.5 Seeds Counted 1174 1124 Percent Top Half 50.2 % 49.6 % Chi - Square <0.1 <0.1 Average Seed Length 500 ± 40 µm 500 ± 60 µm Average Embryo Length 80 ± 20 µm 70 ± 30 µm