SeedGenes Profile: EMB 2754

Gene Information Gene Symbol EMB 2754 Chromosome Locus At4g36630   Alleles in Database 1 Identity Confidence Not Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Putative Vacuolar Protein (VPS39) Function Details Proposed role in vacuolar protein sorting Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Database Release March, 2004 Predicted Sequences Gene: 4790 nucleotides mRNA: 3352 nucleotides Protein:   998 amino acids cDNA Status FLcDNA Available: AY099879 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Medicago truncatula (65%) Model Genomes:   Danio rerio (27%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 246232_at

Mutant Information Allele Symbol emb 2754 Mutant Class Embryo Defective Source of Mutant Meinke / Syngenta Mutant Line Number 120531 Ecotype Columbia Genetic Background qrt ABRC Stock CS24057 Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence Details  (Help) Location of Mutation Exon 1 Terminal Phenotype Cotyledon  Details Nomarski Phenotype   Allelism Evidence Reference Allele Database Release March, 2004 Seed Color Pale Yellow Embryo Color Pale Yellow Percent Mutant Seeds 20.5 % Chi - Square 10.1 Seeds Counted 928 Percent Top Half 58.9 % Chi - Square 6.1 Average Seed Length 510 ± 60 µm Average Embryo Length 140 ± 150 µm