OSU Syngenta vbi

SeedGenes Profile: EMB 2768

Gene Information
Gene Symbol EMB 2768
Chromosome Locus At3g02660   tair
Alleles in Database 2   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function Tyrosine tRNA Synthetase
Function Details Translation in mitochondria and chloroplasts
Gene Evidence
Single Border Recovery
Meinke / Salk (2 alleles)
Database Release October, 2007
Predicted Sequences
Gene:2212 nucleotides
mRNA: 1738 nucleotides
Protein:  511 amino acids
cDNA Status FLcDNA Available: AK221609
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Vitis vinifera (71%)
Model Genomes:  Escherichia coli (43%)
Subcellular Localization TargetP Targeted to Chloroplasts
SUBA
mRNA Expression Data NASCArray Spot History for 258475_at
    
Mutant Information
Allele Symbol emb 2768-1emb 2768-2
Mutant Class Embryo DefectiveEmbryo Defective
Source of Mutant Meinke / SalkMeinke / Salk
Mutant Line Number SALK_016722SALK_045570
Ecotype ColumbiaColumbia
Genetic Background   
ABRC Stock   
Mutagen Treatment T-DNAT-DNA
Insertion Mutant Yes   DetailsYes   Details
Flanking Sequence   
Location of Mutation Exon 1Exon 1
Terminal Phenotype Preglobular  DetailsPreglobular  Details
Nomarski Phenotype Preglobular  ImagesPreglobular  Images
Allelism Evidence Reference AlleleComplementation Test; Sequence Identity
Database Release October, 2007October, 2007
Seed Color WhiteWhite
Embryo Color WhiteWhite
Percent Mutant Seeds 19.9 %21.5 %
Chi - Square 14.16.6
Seeds Counted 10241005
Percent Top Half 54.9 %71.3 %
Chi - Square 2.039.1
Average Seed Length 180 ± 50 µm210 ± 100 µm
Average Embryo Length 15 ± 0 µm20 ± 20 µm