SeedGenes Profile: EMB 2772

Gene Information Gene Symbol EMB 2772 Chromosome Locus At5g14800   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Proline Biosynthesis Function Details Catalyzes final step in proline biosynthesis from glutamate and ornithine Gene Evidence Single Border Recovery Meinke / Salk (2 alleles) Database Release October, 2007 Predicted Sequences Gene: 1878 nucleotides mRNA: 1280 nucleotides Protein:   276 amino acids cDNA Status FLcDNA Available: AF410303 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (92%) Model Genomes:   Drosophila melanogaster (48%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 246594_at

Mutant Information Allele Symbol emb 2772-1 emb 2772-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Salk Meinke / Salk Mutant Line Number SALK_127043 SALK_098189 Ecotype Columbia Columbia Genetic Background     ABRC Stock     Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence     Location of Mutation Exon 1 Intron 5 Terminal Phenotype Preglobular  Details Preglobular  Details Nomarski Phenotype Preglobular  Images Early Globular  Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release October, 2007 October, 2007 Seed Color White White Embryo Color White White Percent Mutant Seeds 21.1 % 24.3 % Chi - Square 9.5 0.3 Seeds Counted 1166 1198 Percent Top Half 61.4 % 56.4 % Chi - Square 12.7 4.7 Average Seed Length 260 ± 60 µm 260 ± 60 µm Average Embryo Length 15 ± 0 µm 15 ± 0 µm