SeedGenes Profile: EMB 2775

Gene Information Gene Symbol EMB 2775 Chromosome Locus At5g27740   Alleles in Database 4   See List OR Show All Identity Confidence Confirmed Alias Symbol EMB 161 (D. Meinke) Complemetation Test Gene Class Embryo Defective Predicted Function DNA Replication Factor (RFC3) Function Details PCNA loading complex factor Gene Evidence Border Recovery D. Meinke (1 allele) Single Border Recovery Meinke / Salk (2 alleles) Database Release October, 2007 Predicted Sequences Gene: 3386 nucleotides mRNA: 1412 nucleotides Protein:   354 amino acids cDNA Status FLcDNA Available: AY074381 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (96%) Model Genomes:   Homo sapiens (53%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 246738_at

Mutant Information Allele Symbol emb 2775-1 Mutant Class Embryo Defective Source of Mutant Meinke / Salk Mutant Line Number SALK_029291 Ecotype Columbia Genetic Background   ABRC Stock   Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence   Location of Mutation Exon 2 Terminal Phenotype Preglobular  Details Nomarski Phenotype Early Globular  Images Allelism Evidence Reference Allele Database Release October, 2007 Seed Color White Embryo Color White Percent Mutant Seeds 23.3 % Chi - Square 1.8 Seeds Counted 1138 Percent Top Half 61.1 % Chi - Square 13.1 Average Seed Length 350 ± 90 µm Average Embryo Length 15 ± 0 µm