SeedGenes Profile: EMB 2775

Gene Information Gene Symbol EMB 2775 Chromosome Locus At5g27740   Alleles in Database 4   See List OR Show All Identity Confidence Confirmed Alias Symbol EMB 161 (D. Meinke) Complemetation Test Gene Class Embryo Defective Predicted Function DNA Replication Factor (RFC3) Function Details PCNA loading complex factor Gene Evidence Border Recovery D. Meinke (1 allele) Single Border Recovery Meinke / Salk (2 alleles) Database Release October, 2007 Predicted Sequences Gene: 3386 nucleotides mRNA: 1412 nucleotides Protein:   354 amino acids cDNA Status FLcDNA Available: AY074381 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (96%) Model Genomes:   Homo sapiens (53%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 246738_at

Mutant Information Allele Symbol emb 2775-1 emb 2775-2 emb 161-1 emb 161-2 Mutant Class Embryo Defective Embryo Defective Embryo Defective Embryo Defective Source of Mutant Meinke / Salk Meinke / Salk Meinke / DuPont Meinke / DuPont Mutant Line Number SALK_029291 SALK_022350 5575 2077 Ecotype Columbia Columbia Wassilewskija Wassilewskija Genetic Background         ABRC Stock         Mutagen Treatment T-DNA T-DNA T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details No Yes   Details Flanking Sequence         Location of Mutation Exon 2 Exon 7   Intron 1 Terminal Phenotype Preglobular  Details Preglobular  Details Preglobular Preglobular Nomarski Phenotype Early Globular  Images Preglobular  Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Complementation Test Complementation Test; Sequence Identity Database Release October, 2007 October, 2007 December, 2010 December, 2010 Seed Color White White     Embryo Color White White     Percent Mutant Seeds 23.3 % 23.7 % 21.1 % 24.5 % Chi - Square 1.8 1.1     Seeds Counted 1138 1169     Percent Top Half 61.1 % 53.1 % 51.2 %   Chi - Square 13.1 1.0     Average Seed Length 350 ± 90 µm 300 ± 70 µm     Average Embryo Length 15 ± 0 µm 15 ± 0 µm