OSU Syngenta vbi

SeedGenes Profile: EMB 2775

Gene Information
Gene Symbol EMB 2775
Chromosome Locus At5g27740   tair
Alleles in Database 4   See List OR Show All
Identity Confidence Confirmed
Alias Symbol EMB 161 (D. Meinke) Complemetation Test
Gene Class Embryo Defective
Predicted Function DNA Replication Factor (RFC3)
Function Details PCNA loading complex factor
Gene Evidence
Border Recovery
D. Meinke (1 allele)
Single Border Recovery
Meinke / Salk (2 alleles)
Database Release October, 2007
Predicted Sequences
Gene:3386 nucleotides
mRNA: 1412 nucleotides
Protein:  354 amino acids
cDNA Status FLcDNA Available: AY074381
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Brassica napus (96%)
Model Genomes:  Homo sapiens (53%)
Subcellular Localization TargetP Not Targeted to Cell Compartment
mRNA Expression Data NASCArray Spot History for 246738_at
Mutant Information
Allele Symbol emb 2775-2
Mutant Class Embryo Defective
Source of Mutant Meinke / Salk
Mutant Line Number SALK_022350
Ecotype Columbia
Genetic Background  
ABRC Stock  
Mutagen Treatment T-DNA
Insertion Mutant Yes   Details
Flanking Sequence  
Location of Mutation Exon 7
Terminal Phenotype Preglobular  Details
Nomarski Phenotype Preglobular  Images
Allelism Evidence Complementation Test; Sequence Identity
Database Release October, 2007
Seed Color White
Embryo Color White
Percent Mutant Seeds 23.7 %
Chi - Square 1.1
Seeds Counted 1169
Percent Top Half 53.1 %
Chi - Square 1.0
Average Seed Length 300 ± 70 µm
Average Embryo Length 15 ± 0 µm