SeedGenes Profile: EMB 2804

Gene Information Gene Symbol EMB 2804 Chromosome Locus At3g10220   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Tubulin Folding Cofactor B Function Details Microtubule polymerization Gene Evidence Border Recovery Meinke / Salk (1 allele) Border Recovery Meinke / SAIL (1 allele) Database Release December, 2010 Predicted Sequences Gene: 2475 nucleotides mRNA: 1058 nucleotides Protein:   243 amino acids cDNA Status FLcDNA Available: AK176266 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (88%) Model Genomes:   Caenorhabditis elegans (40%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 259141_at

Mutant Information Allele Symbol emb 2804-1 emb 2804-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Salk Meinke / SAIL Mutant Line Number SALK_019471 SAIL_169_D05 Ecotype Columbia Columbia Genetic Background     ABRC Stock     Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence     Location of Mutation Intron 7 Intron 5 Terminal Phenotype Preglobular  Details Preglobular  Details Nomarski Phenotype   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release December, 2010 December, 2010 Seed Color White White Embryo Color White White Percent Mutant Seeds 19.9 % 26.5 % Chi - Square 3.3 0.2 Seeds Counted 241 151 Percent Top Half 35.4 % 45.0 % Chi - Square 4.1 0.2 Average Seed Length 340 ± 70 µm 340 ± 70 µm Average Embryo Length 15 ± 0 µm 15 ± 0 µm