SeedGenes Profile: EMB 2813

Gene Information Gene Symbol EMB 2813 Chromosome Locus At1g67320   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function DNA Polymerase Alpha (POLA3) Function Details DNA replication Gene Evidence Border Recovery Meinke / Salk (1 allele) Border Recovery Meinke / SAIL (1 allele) Database Release December, 2010 Predicted Sequences Gene: 3581 nucleotides mRNA: 1484 nucleotides Protein:   449 amino acids cDNA Status FLcDNA Available: BX815018 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Medicago truncatula (71%) Model Genomes:   Danio rerio (41%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 264969_at

Mutant Information Allele Symbol emb 2813-1 emb 2813-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Salk Meinke / SAIL Mutant Line Number SALK_045856 SAIL_720_B06 Ecotype Columbia Columbia Genetic Background     ABRC Stock     Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence     Location of Mutation Exon 8 Intron 12 Terminal Phenotype Preglobular Preglobular Nomarski Phenotype Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release December, 2010 December, 2010 Seed Color     Embryo Color     Percent Mutant Seeds 13.5 % 36.6 % Chi - Square 14.8 117.0 Seeds Counted 208 1558 Percent Top Half     Chi - Square     Average Seed Length     Average Embryo Length