SeedGenes Profile: EMB 2814

Gene Information Gene Symbol EMB 2814 Chromosome Locus At1g67630   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function DNA Polymerase Alpha (POLA2) Function Details DNA replication Gene Evidence Border Recovery Meinke / Salk (1 allele) Border Recovery Meinke / Riken (1 allele) Database Release December, 2010 Predicted Sequences Gene: 3967 nucleotides mRNA: 2115 nucleotides Protein:   620 amino acids cDNA Status FLcDNA Available: BX816039 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Spruce (50%) Model Genomes:   Danio rerio (37%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 260192_at

Mutant Information Allele Symbol emb 2814-2 Mutant Class Embryo Defective Source of Mutant Meinke / Riken Mutant Line Number RATM16-2074-1 Ecotype Nossen Genetic Background   ABRC Stock   Mutagen Treatment Transposon Insertion Mutant Yes   Details Flanking Sequence   Location of Mutation Exon 4 Terminal Phenotype Preglobular Nomarski Phenotype   Allelism Evidence Complementation Test; Sequence Identity Database Release December, 2010 Seed Color   Embryo Color   Percent Mutant Seeds 23.4 % Chi - Square 0.2 Seeds Counted 107 Percent Top Half   Chi - Square   Average Seed Length   Average Embryo Length