SeedGenes Profile: EMB 2814

Gene Information Gene Symbol EMB 2814 Chromosome Locus At1g67630   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function DNA Polymerase Alpha (POLA2) Function Details DNA replication Gene Evidence Border Recovery Meinke / Salk (1 allele) Border Recovery Meinke / Riken (1 allele) Database Release December, 2010 Predicted Sequences Gene: 3967 nucleotides mRNA: 2115 nucleotides Protein:   620 amino acids cDNA Status FLcDNA Available: BX816039 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Spruce (50%) Model Genomes:   Danio rerio (37%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 260192_at

Mutant Information Allele Symbol emb 2814-1 emb 2814-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Salk Meinke / Riken Mutant Line Number SALK_064094 RATM16-2074-1 Ecotype Columbia Nossen Genetic Background     ABRC Stock     Mutagen Treatment T-DNA Transposon Insertion Mutant Yes   Details Yes   Details Flanking Sequence     Location of Mutation Intron 7 Exon 4 Terminal Phenotype Preglobular Preglobular Nomarski Phenotype Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release December, 2010 December, 2010 Seed Color     Embryo Color     Percent Mutant Seeds 23.8 % 23.4 % Chi - Square 0.3 0.2 Seeds Counted 421 107 Percent Top Half     Chi - Square     Average Seed Length     Average Embryo Length