OSU Syngenta vbi

SeedGenes Profile: EMB 3004

Gene Information
Gene Symbol EMB 3004
Chromosome Locus At3g06350   tair
Alleles in Database 2   See List OR Show All
Identity Confidence Confirmed
Alias Symbol MEE 32 (Sundaresan) Sequence Identity
Gene Class Embryo Defective
Predicted Function Dehydroquinate Dehydratase; Shikimate Dehydrogenase
Function Details Chorismate biosynthesis
Gene Evidence
Single Border Recovery
Meinke / Genoplante (1 allele)
Border Recovery
Singh / EXOTIC (1 allele)
Database Release September, 2004
Predicted Sequences
Gene:3450 nucleotides
mRNA: 2096 nucleotides
Protein:  603 amino acids
cDNA Status FLcDNA Available: BX823611
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Brassica napus (88%)
Model Genomes:   None
Subcellular Localization TargetP Targeted to Chloroplasts
mRNA Expression Data NASCArray Spot History for 258908_at
Mutant Information
Allele Symbol emb 3004-1
Mutant Class Embryo Defective
Source of Mutant Meinke / Genoplante
Mutant Line Number FBU3
Ecotype Wassilewskija
Genetic Background  
ABRC Stock  
Mutagen Treatment T-DNA
Insertion Mutant Yes   Details
Flanking Sequence  
Location of Mutation 5` UTR to Exon 1
Terminal Phenotype Cotyledon  Details
Nomarski Phenotype   Images
Allelism Evidence Reference Allele
Database Release September, 2004
Seed Color Pale Yellow
Embryo Color Pale Yellow
Percent Mutant Seeds 24.6 %
Chi - Square 0.1
Seeds Counted 1017
Percent Top Half 56.0 %
Chi - Square 3.6
Average Seed Length 460 ± 60 µm
Average Embryo Length 140 ± 100 µm