SeedGenes Profile: EMB 3116

Gene Information Gene Symbol EMB 3116 Chromosome Locus At2g44190   Alleles in Database 5   See List OR Show All Identity Confidence Confirmed Alias Symbol EDE 1 (M. Hills) Sequence Identity Gene Class Embryo Defective Predicted Function Novel Microtubule Associated Protein Function Details Endosperm nuclear division and cellularization Gene Evidence Sequenced Mutant Allele M. Hills (1 allele) Molecular Complementation M. Hills (1 allele) Border Recovery Hills / Salk (2 alleles) Border Recovery Myouga / Riken (2 alleles) Database Release December, 2010 Predicted Sequences Gene: 2307 nucleotides mRNA: 1745 nucleotides Protein:   474 amino acids cDNA Status FLcDNA Available: BX818775 Top BLAST Hits Arabidopsis: AT3G60000.1 (57%) Plant ESTs: Brassica napus (76%) Model Genomes:   None Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 267232_at

Mutant Information Allele Symbol emb 3116-2 Mutant Class Embryo Defective Source of Mutant Myouga / Riken Mutant Line Number RATM15-2706-1 Ecotype Nossen Genetic Background   ABRC Stock   Mutagen Treatment Transposon Insertion Mutant Yes   Details Flanking Sequence   Location of Mutation Exon 1 Terminal Phenotype Preglobular Nomarski Phenotype Globular Allelism Evidence Complementation Test; Sequence Identity Database Release December, 2010 Seed Color   Embryo Color   Percent Mutant Seeds 22.3 % Chi - Square 0.7 Seeds Counted 188 Percent Top Half   Chi - Square   Average Seed Length   Average Embryo Length