SeedGenes Profile: EMB 3116

Gene Information Gene Symbol EMB 3116 Chromosome Locus At2g44190   Alleles in Database 5   See List OR Show All Identity Confidence Confirmed Alias Symbol EDE 1 (M. Hills) Sequence Identity Gene Class Embryo Defective Predicted Function Novel Microtubule Associated Protein Function Details Endosperm nuclear division and cellularization Gene Evidence Sequenced Mutant Allele M. Hills (1 allele) Molecular Complementation M. Hills (1 allele) Border Recovery Hills / Salk (2 alleles) Border Recovery Myouga / Riken (2 alleles) Database Release December, 2010 Predicted Sequences Gene: 2307 nucleotides mRNA: 1745 nucleotides Protein:   474 amino acids cDNA Status FLcDNA Available: BX818775 Top BLAST Hits Arabidopsis: AT3G60000.1 (57%) Plant ESTs: Brassica napus (76%) Model Genomes:   None Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 267232_at

Mutant Information Allele Symbol emb 3116-1 emb 3116-2 ede 1-1 ede 1-2 ede 1-3 Mutant Class Embryo Defective Embryo Defective Embryo Defective Embryo Defective Embryo Defective Source of Mutant Myouga / Riken Myouga / Riken C. Benning Hills / Salk Hills / Salk Mutant Line Number RATM12-5223-1 RATM15-2706-1   SALK_047950 SALK_048191 Ecotype Nossen Nossen Columbia Columbia Columbia Genetic Background           ABRC Stock           Mutagen Treatment Transposon Transposon EMS T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details No Yes   Details Yes   Details Flanking Sequence           Location of Mutation Exon 1 Exon 1 Exon 3 Exon 1 Intron 3 Terminal Phenotype Preglobular Preglobular Cotyledon Transition Transition Nomarski Phenotype Globular Globular Allelism Evidence Reference Allele Complementation Test; Sequence Identity Sequence Identity Complementation Test; Sequence Identity Complementation Test; Sequence Identity Database Release December, 2010 December, 2010 December, 2010 December, 2010 December, 2010 Seed Color           Embryo Color           Percent Mutant Seeds 24.5 % 22.3 %       Chi - Square <0.1 0.7       Seeds Counted 269 188       Percent Top Half           Chi - Square           Average Seed Length           Average Embryo Length