SeedGenes Profile: HCS 1

Gene Information Gene Symbol HCS 1 Chromosome Locus At2g25710   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Holocarboxylase Synthetase Function Details Biotin attachment to carboxylases and decarboxylases Gene Evidence Border Recovery Alban / SAIL (1 allele) Border Recovery Alban / FLAG (1 allele) Database Release December, 2010 Predicted Sequences Gene: 2720 nucleotides mRNA: 1481 nucleotides Protein:   367 amino acids cDNA Status FLcDNA Available: BX821008 Top BLAST Hits Arabidopsis: AT1G37150.1 (89%) Plant ESTs: Brassica napus (84%) Model Genomes:   Homo sapiens (31%) Subcellular Localization TargetP Targeted to Mitochondria SUBA mRNA Expression Data NASCArray Spot History for 265887_at

Mutant Information Allele Symbol hcs 1-1 hcs 1-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Alban / SAIL Alban / FLAG Mutant Line Number SAIL_1277_E03 FLAG_486E08 Ecotype Columbia Wassilewskija Genetic Background     ABRC Stock     Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence     Location of Mutation Exon 2 Intron 9 Terminal Phenotype Preglobular Preglobular Nomarski Phenotype Allelism Evidence Reference Allele Sequence Identity Database Release December, 2010 December, 2010 Seed Color     Embryo Color     Percent Mutant Seeds     Chi - Square     Seeds Counted     Percent Top Half     Chi - Square     Average Seed Length     Average Embryo Length