SeedGenes Profile: HISN 2

Gene Information Gene Symbol HISN 2 Chromosome Locus At1g31860   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Histidine Biosynthesis Function Details Catalyzes the second step in histidine biosynthesis Gene Evidence Single Border Recovery Meinke / Salk (3 alleles) Database Release October, 2007 Predicted Sequences Gene: 2500 nucleotides mRNA: 1065 nucleotides Protein:   281 amino acids cDNA Status FLcDNA Available: AY086514 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (86%) Model Genomes:   None Subcellular Localization TargetP Targeted to Mitochondria SUBA mRNA Expression Data NASCArray Spot History for 246265_at

Mutant Information Allele Symbol hisn 2-1 hisn 2-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Salk Meinke / Salk Mutant Line Number SALK_047603 SALK_143868 Ecotype Columbia Columbia Genetic Background     ABRC Stock     Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence     Location of Mutation Exon 5 Exon 5 Terminal Phenotype Preglobular  Details Preglobular  Details Nomarski Phenotype Preglobular  Images Preglobular  Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release October, 2007 October, 2007 Seed Color White White Embryo Color White White Percent Mutant Seeds 16.0 % 16.0 % Chi - Square 38.5 72.7 Seeds Counted 898 1672 Percent Top Half 71.5 % 69.3 % Chi - Square 26.7 39.7 Average Seed Length 320 ± 80 µm 220 ± 80 µm Average Embryo Length 20 ± 20 µm 15 ± 0 µm