SeedGenes Profile: HISN 3

Gene Information Gene Symbol HISN 3 Chromosome Locus At2g36230   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol APG 10 (K. Shinozaki) Sequence Identity Gene Class Embryo Defective Predicted Function Histidine Biosynthesis Function Details Catalyzes the third step in histidine biosynthesis Gene Evidence Border Recovery Shinozaki / Riken (1 allele) Single Border Recovery Meinke / Salk (2 alleles) Database Release October, 2007 Predicted Sequences Gene: 1845 nucleotides mRNA: 1128 nucleotides Protein:   304 amino acids cDNA Status FLcDNA Available: AB006139 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (91%) Model Genomes:   Schizosaccharomyces pombe (50%) Subcellular Localization TargetP Targeted to Mitochondria SUBA mRNA Expression Data NASCArray Spot History for 263902_at

Mutant Information Allele Symbol hisn 3-2 Mutant Class Embryo Defective Source of Mutant Meinke / Salk Mutant Line Number SALK_097704 Ecotype Columbia Genetic Background   ABRC Stock   Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence   Location of Mutation Intron 4 Terminal Phenotype Preglobular  Details Nomarski Phenotype Preglobular  Images Allelism Evidence Complementation Test; Sequence Identity Database Release October, 2007 Seed Color White Embryo Color White Percent Mutant Seeds 20.7 % Chi - Square 20.6 Seeds Counted 2092 Percent Top Half 70.4 % Chi - Square 72.3 Average Seed Length 280 ± 80 µm Average Embryo Length 20 ± 10 µm