OSU Syngenta vbi

SeedGenes Profile: HISN 3

Gene Information
Gene Symbol HISN 3
Chromosome Locus At2g36230   tair
Alleles in Database 3   See List OR Show All
Identity Confidence Confirmed
Alias Symbol APG 10 (K. Shinozaki) Sequence Identity
Gene Class Embryo Defective
Predicted Function Histidine Biosynthesis
Function Details Catalyzes the third step in histidine biosynthesis
Gene Evidence
Border Recovery
Shinozaki / Riken (1 allele)
Single Border Recovery
Meinke / Salk (2 alleles)
Database Release October, 2007
Predicted Sequences
Gene:1845 nucleotides
mRNA: 1128 nucleotides
Protein:  304 amino acids
cDNA Status FLcDNA Available: AB006139
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Brassica napus (91%)
Model Genomes:  Schizosaccharomyces pombe (50%)
Subcellular Localization TargetP Targeted to Mitochondria
SUBA
mRNA Expression Data NASCArray Spot History for 263902_at
    
Mutant Information
Allele Symbol hisn 3-1hisn 3-2apg 10-2
Mutant Class Embryo DefectiveEmbryo DefectiveEmbryo Defective
Source of Mutant Meinke / SalkMeinke / SalkShinozaki / Riken
Mutant Line Number SALK_041416SALK_097704DS1517671
Ecotype ColumbiaColumbiaNossen
Genetic Background    
ABRC Stock    
Mutagen Treatment T-DNAT-DNATransposon
Insertion Mutant Yes   DetailsYes   DetailsYes   Details
Flanking Sequence    
Location of Mutation Exon 2Intron 4Intron 6
Terminal Phenotype Preglobular  DetailsPreglobular  DetailsNot Available
Nomarski Phenotype Preglobular  ImagesPreglobular  Images
Allelism Evidence Reference AlleleComplementation Test; Sequence IdentitySU
Database Release October, 2007October, 2007October, 2007
Seed Color WhiteWhite 
Embryo Color WhiteWhite 
Percent Mutant Seeds 21.7 %20.7 % 
Chi - Square 12.820.6 
Seeds Counted 21682092 
Percent Top Half 72.9 %70.4 % 
Chi - Square 99.372.3 
Average Seed Length 270 ± 80 µm280 ± 80 µm 
Average Embryo Length 20 ± 10 µm20 ± 10 µm