Aug 17, 2018 4:41 PM
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Database Query
SeedGenes Profile: HISN 3
Gene Information
Gene Symbol
HISN 3
Chromosome Locus
At2g36230
Alleles in Database
3
See List
OR
Show All
Identity Confidence
Confirmed
Alias Symbol
APG 10
(K. Shinozaki) Sequence Identity
Gene Class
Embryo Defective
Predicted Function
Histidine Biosynthesis
Function Details
Catalyzes the third step in histidine biosynthesis
Gene Evidence
Border Recovery
Shinozaki / Riken (1 allele)
Single Border Recovery
Meinke / Salk (2 alleles)
Database Release
October, 2007
Predicted Sequences
Gene:
1845 nucleotides
mRNA:
1128 nucleotides
Protein:
304 amino acids
cDNA Status
FLcDNA Available:
AB006139
Top BLAST Hits
Arabidopsis
:
Only self hit
Plant ESTs:
Brassica napus (91%)
Model Genomes:
Schizosaccharomyces pombe (50%)
Subcellular Localization
TargetP
Targeted to Mitochondria
SUBA
mRNA Expression Data
NASCArray Spot History for
263902_at
Mutant Information
Allele Symbol
hisn 3-1
hisn 3-2
apg 10-2
Mutant Class
Embryo Defective
Embryo Defective
Embryo Defective
Source of Mutant
Meinke / Salk
Meinke / Salk
Shinozaki / Riken
Mutant Line Number
SALK_041416
SALK_097704
DS1517671
Ecotype
Columbia
Columbia
Nossen
Genetic Background
ABRC Stock
Mutagen Treatment
T-DNA
T-DNA
Transposon
Insertion Mutant
Yes
Details
Yes
Details
Yes
Details
Flanking Sequence
Location of Mutation
Exon 2
Intron 4
Intron 6
Terminal Phenotype
Preglobular
Details
Preglobular
Details
Not Available
Nomarski Phenotype
Preglobular
Images
Preglobular
Images
Allelism Evidence
Reference Allele
Complementation Test; Sequence Identity
SU
Database Release
October, 2007
October, 2007
October, 2007
Seed Color
White
White
Embryo Color
White
White
Percent Mutant Seeds
21.7 %
20.7 %
Chi - Square
12.8
20.6
Seeds Counted
2168
2092
Percent Top Half
72.9 %
70.4 %
Chi - Square
99.3
72.3
Average Seed Length
270 ± 80 µm
280 ± 80 µm
Average Embryo Length
20 ± 10 µm
20 ± 10 µm