SeedGenes Profile: HISN 4

Gene Information Gene Symbol HISN 4 Chromosome Locus At4g26900   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Histidine Biosynthesis Function Details Catalyzes the fourth step in histidine biosynthesis Gene Evidence Single Border Recovery Meinke / Salk (2 alleles) Single Border Recovery Meinke / SAIL (1 allele) Database Release October, 2007 Predicted Sequences Gene: 4378 nucleotides mRNA: 2062 nucleotides Protein:   592 amino acids cDNA Status FLcDNA Available: AK118498 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (92%) Model Genomes:   Neurospora crassa (53%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 253921_at

Mutant Information Allele Symbol hisn 4-2 Mutant Class Embryo Defective Source of Mutant Meinke / Salk Mutant Line Number SALK_002283 Ecotype Columbia Genetic Background   ABRC Stock   Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence   Location of Mutation Exon 15 Terminal Phenotype Preglobular  Details Nomarski Phenotype Early Globular  Images Allelism Evidence Complementation Test; Sequence Identity Database Release October, 2007 Seed Color White Embryo Color White Percent Mutant Seeds 23.4 % Chi - Square 2.8 Seeds Counted 2114 Percent Top Half 65.8 % Chi - Square 49.8 Average Seed Length 330 ± 110 µm Average Embryo Length 20 ± 20 µm