SeedGenes Profile: HISN 4

Gene Information Gene Symbol HISN 4 Chromosome Locus At4g26900   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Histidine Biosynthesis Function Details Catalyzes the fourth step in histidine biosynthesis Gene Evidence Single Border Recovery Meinke / Salk (2 alleles) Single Border Recovery Meinke / SAIL (1 allele) Database Release October, 2007 Predicted Sequences Gene: 4378 nucleotides mRNA: 2062 nucleotides Protein:   592 amino acids cDNA Status FLcDNA Available: AK118498 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (92%) Model Genomes:   Neurospora crassa (53%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 253921_at

Mutant Information Allele Symbol hisn 4-1 hisn 4-2 hisn 4-3 Mutant Class Embryo Defective Embryo Defective Embryo Defective Source of Mutant Meinke / Salk Meinke / Salk Meinke / SAIL Mutant Line Number SALK_041907 SALK_002283 SAIL_556_C04 Ecotype Columbia Columbia Columbia Genetic Background       ABRC Stock       Mutagen Treatment T-DNA T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Yes   Details Flanking Sequence       Location of Mutation Exon 1 Exon 15 Intron 17 Terminal Phenotype Preglobular  Details Preglobular  Details Preglobular  Details Nomarski Phenotype Preglobular  Images Early Globular  Images Preglobular  Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Complementation Test; Sequence Identity Database Release October, 2007 October, 2007 October, 2007 Seed Color White White White Embryo Color White White White Percent Mutant Seeds 21.8 % 23.4 % 23.5 % Chi - Square 10.8 2.8 2.5 Seeds Counted 2059 2114 2161 Percent Top Half 64.7 % 65.8 % 58.7 % Chi - Square 38.7 49.8 15.2 Average Seed Length 380 ± 110 µm 330 ± 110 µm 330 ± 120 µm Average Embryo Length 20 ± 20 µm 20 ± 20 µm 20 ± 30 µm