SeedGenes Profile: HISN 6A

Gene Information Gene Symbol HISN 6A Chromosome Locus At5g10330   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol HPA 1 (X. Mo; P. Wu) Sequence Identity EMB 2196 (D. Meinke) Alternate Name Gene Class Embryo Defective Predicted Function Histidinol Phosphate Aminotransferase Function Details Histidine biosynthesis Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Border Recovery Meinke / SAIL (1 allele) Database Release March, 2003 Predicted Sequences Gene: 2276 nucleotides mRNA: 1611 nucleotides Protein:   417 amino acids cDNA Status FLcDNA Available: AY050832 Top BLAST Hits Arabidopsis: AT1G71920.1 (99%) Plant ESTs: Brassica napus (88%) Model Genomes:   Neurospora crassa (35%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 260172_s_at

Mutant Information Allele Symbol hisn 6a-2 Mutant Class Embryo Defective Source of Mutant Meinke / SAIL Mutant Line Number SAIL_750_F1 Ecotype Columbia Genetic Background   ABRC Stock   Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence   Location of Mutation Exon 7 Terminal Phenotype Preglobular  Details Nomarski Phenotype Preglobular Allelism Evidence Complementation Test; Sequence Identity Database Release October, 2007 Seed Color White Embryo Color White Percent Mutant Seeds 25.4 % Chi - Square 0.1 Seeds Counted 1228 Percent Top Half 59.2 % Chi - Square 10.8 Average Seed Length 370 ± 100 µm Average Embryo Length 20 ± 20 µm