SeedGenes Profile: HISN 6A

Gene Information Gene Symbol HISN 6A Chromosome Locus At5g10330   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol HPA 1 (X. Mo; P. Wu) Sequence Identity EMB 2196 (D. Meinke) Alternate Name Gene Class Embryo Defective Predicted Function Histidinol Phosphate Aminotransferase Function Details Histidine biosynthesis Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Border Recovery Meinke / SAIL (1 allele) Database Release March, 2003 Predicted Sequences Gene: 2276 nucleotides mRNA: 1611 nucleotides Protein:   417 amino acids cDNA Status FLcDNA Available: AY050832 Top BLAST Hits Arabidopsis: AT1G71920.1 (99%) Plant ESTs: Brassica napus (88%) Model Genomes:   Neurospora crassa (35%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 260172_s_at

Mutant Information Allele Symbol hisn 6a-1 hisn 6a-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / SAIL Mutant Line Number 69725 SAIL_750_F1 Ecotype Columbia Columbia Genetic Background qrt   ABRC Stock CS16211   Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help)   Location of Mutation Exon 2 Exon 7 Terminal Phenotype Preglobular  Details Preglobular  Details Nomarski Phenotype Preglobular  Images Preglobular Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release March, 2003 October, 2007 Seed Color White White Embryo Color White White Percent Mutant Seeds 25.0 % 25.4 % Chi - Square <0.1 0.1 Seeds Counted 1053 1228 Percent Top Half 54.4 % 59.2 % Chi - Square 2.0 10.8 Average Seed Length 340 ± 50 µm 370 ± 100 µm Average Embryo Length 15 ± 0 µm 20 ± 20 µm