SeedGenes Profile: HYD 1

Gene Information Gene Symbol HYD 1 Chromosome Locus At1g20050   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Sterol Isomerase Function Details Catalyzes sterol biosynthetic step downstream of FACKEL Gene Evidence Sequenced Mutant Allele K. Lindsey (1 allele) Molecular Complementation K. Lindsey (1 allele) Border Recovery K. Lindsey (1 allele) Database Release September, 2002 Predicted Sequences Gene: 1277 nucleotides mRNA: 973 nucleotides Protein:   223 amino acids cDNA Status FLcDNA Available: AY086087 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (83%) Model Genomes:   None Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 261228_at

Mutant Information Allele Symbol hyd 1-2 hyd 1-1 Mutant Class Embryo Defective Embryo Defective Source of Mutant K. Lindsey K. Lindsey Mutant Line Number     Ecotype Wassilewskija Uncertain Genetic Background     ABRC Stock     Mutagen Treatment T-DNA Uncertain Insertion Mutant Yes   Details No Flanking Sequence     Location of Mutation Intron 1 Exon 2 Terminal Phenotype Cotyledon  Details Cotyledon  Details Nomarski Phenotype   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release September, 2002 September, 2002 Seed Color Green Green Embryo Color Green Green Percent Mutant Seeds 28.5 % 26.5 % Chi - Square 8.1 1.3 Seeds Counted 1209 1074 Percent Top Half 54.8 % 58.2 % Chi - Square 3.2 7.8 Average Seed Length 520 ± 70 µm 520 ± 70 µm Average Embryo Length 290 ± 70 µm 330 ± 80 µm