OSU Syngenta vbi

SeedGenes Profile: HYD 1

Gene Information
Gene Symbol HYD 1
Chromosome Locus At1g20050   tair
Alleles in Database 2   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function Sterol Isomerase
Function Details Catalyzes sterol biosynthetic step downstream of FACKEL
Gene Evidence
Sequenced Mutant Allele
K. Lindsey (1 allele)
Molecular Complementation
K. Lindsey (1 allele)
Border Recovery
K. Lindsey (1 allele)
Database Release September, 2002
Predicted Sequences
Gene:1277 nucleotides
mRNA: 973 nucleotides
Protein:  223 amino acids
cDNA Status FLcDNA Available: AY086087
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Brassica napus (83%)
Model Genomes:   None
Subcellular Localization TargetP Not Targeted to Cell Compartment
SUBA
mRNA Expression Data NASCArray Spot History for 261228_at
    
Mutant Information
Allele Symbol hyd 1-2hyd 1-1
Mutant Class Embryo DefectiveEmbryo Defective
Source of Mutant K. LindseyK. Lindsey
Mutant Line Number   
Ecotype WassilewskijaUncertain
Genetic Background   
ABRC Stock   
Mutagen Treatment T-DNAUncertain
Insertion Mutant Yes   DetailsNo
Flanking Sequence   
Location of Mutation Intron 1Exon 2
Terminal Phenotype Cotyledon  DetailsCotyledon  Details
Nomarski Phenotype   Images  Images
Allelism Evidence Reference AlleleComplementation Test; Sequence Identity
Database Release September, 2002September, 2002
Seed Color GreenGreen
Embryo Color GreenGreen
Percent Mutant Seeds 28.5 %26.5 %
Chi - Square 8.11.3
Seeds Counted 12091074
Percent Top Half 54.8 %58.2 %
Chi - Square 3.27.8
Average Seed Length 520 ± 70 µm520 ± 70 µm
Average Embryo Length 290 ± 70 µm330 ± 80 µm