SeedGenes Profile: ISE 2

Gene Information Gene Symbol ISE 2 Chromosome Locus At1g70070   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol EMB 25 (D. Meinke) Sequence Identity PDE 317 (D. Meinke) Sequence Identity EMB 25 (D. Meinke) Complemetation Test PDE 317 (D. Meinke) Complemetation Test Gene Class Embryo Defective Predicted Function DEAD Box Helicase Function Details Putative RNA binding protein; Plasmodesmata function Gene Evidence Sequenced Mutant Allele P. Zambryski (2 alleles) Double Border Recovery Meinke / Syngenta (1 allele) Database Release September, 2003 Predicted Sequences Gene: 4181 nucleotides mRNA: 3564 nucleotides Protein:   1171 amino acids cDNA Status EST Identified Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Rice (Oryza sativa) (66%) Model Genomes:   Synechocystis (32%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 264748_at

Mutant Information Allele Symbol ise 2-1 emb 25 Mutant Class Embryo Defective Embryo Defective Source of Mutant Zambryski / Lehle D. Meinke Mutant Line Number   115J-4A Ecotype Landsberg erecta Columbia Genetic Background     ABRC Stock     Mutagen Treatment EMS EMS Insertion Mutant No No Flanking Sequence     Location of Mutation Exon 1 Exon 2 Terminal Phenotype Cotyledon Cotyledon Nomarski Phenotype Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release October, 2007 October, 2007 Seed Color     Embryo Color     Percent Mutant Seeds     Chi - Square     Seeds Counted     Percent Top Half     Chi - Square     Average Seed Length     Average Embryo Length