OSU Syngenta vbi

SeedGenes Profile: ISE 2

Gene Information
Gene Symbol ISE 2
Chromosome Locus At1g70070   tair
Alleles in Database 3   See List OR Show All
Identity Confidence Confirmed
Alias Symbol EMB 25 (D. Meinke) Sequence Identity
PDE 317 (D. Meinke) Sequence Identity
EMB 25 (D. Meinke) Complemetation Test
PDE 317 (D. Meinke) Complemetation Test
Gene Class Embryo Defective
Predicted Function DEAD Box Helicase
Function Details Putative RNA binding protein; Plasmodesmata function
Gene Evidence
Sequenced Mutant Allele
P. Zambryski (2 alleles)
Double Border Recovery
Meinke / Syngenta (1 allele)
Database Release September, 2003
Predicted Sequences
Gene:4181 nucleotides
mRNA: 3564 nucleotides
Protein:  1171 amino acids
cDNA Status EST Identified
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Rice (Oryza sativa) (66%)
Model Genomes:  Synechocystis (32%)
Subcellular Localization TargetP Targeted to Chloroplasts
SUBA
mRNA Expression Data NASCArray Spot History for 264748_at
    
Mutant Information
Allele Symbol ise 2-1emb 25pde 317
Mutant Class Embryo DefectiveEmbryo DefectiveSeed Pigment
Source of Mutant Zambryski / LehleD. MeinkeMeinke / Syngenta
Mutant Line Number  115J-4A73215
Ecotype Landsberg erectaColumbiaColumbia
Genetic Background   qrt
ABRC Stock   CS16227
Mutagen Treatment EMSEMST-DNA
Insertion Mutant NoNoYes   Details
Flanking Sequence   Details  (Help)
Location of Mutation Exon 1Exon 2Exon 6
Terminal Phenotype CotyledonCotyledonPigment Defective
Nomarski Phenotype
Allelism Evidence Reference AlleleComplementation Test; Sequence IdentityComplementation Test; Sequence Identity
Database Release October, 2007October, 2007September, 2003
Seed Color   White
Embryo Color   White
Percent Mutant Seeds   29.0 %
Chi - Square   4.7
Seeds Counted   563
Percent Top Half   46.6 %
Chi - Square   0.7
Average Seed Length    
Average Embryo Length