SeedGenes Profile: ISE 2

Gene Information Gene Symbol ISE 2 Chromosome Locus At1g70070   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol EMB 25 (D. Meinke) Sequence Identity PDE 317 (D. Meinke) Sequence Identity EMB 25 (D. Meinke) Complemetation Test PDE 317 (D. Meinke) Complemetation Test Gene Class Embryo Defective Predicted Function DEAD Box Helicase Function Details Putative RNA binding protein; Plasmodesmata function Gene Evidence Sequenced Mutant Allele P. Zambryski (2 alleles) Double Border Recovery Meinke / Syngenta (1 allele) Database Release September, 2003 Predicted Sequences Gene: 4181 nucleotides mRNA: 3564 nucleotides Protein:   1171 amino acids cDNA Status EST Identified Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Rice (Oryza sativa) (66%) Model Genomes:   Synechocystis (32%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 264748_at

Mutant Information Allele Symbol ise 2-1 emb 25 pde 317 Mutant Class Embryo Defective Embryo Defective Seed Pigment Source of Mutant Zambryski / Lehle D. Meinke Meinke / Syngenta Mutant Line Number   115J-4A 73215 Ecotype Landsberg erecta Columbia Columbia Genetic Background     qrt ABRC Stock     CS16227 Mutagen Treatment EMS EMS T-DNA Insertion Mutant No No Yes   Details Flanking Sequence     Details  (Help) Location of Mutation Exon 1 Exon 2 Exon 6 Terminal Phenotype Cotyledon Cotyledon Pigment Defective Nomarski Phenotype Allelism Evidence Reference Allele Complementation Test; Sequence Identity Complementation Test; Sequence Identity Database Release October, 2007 October, 2007 September, 2003 Seed Color     White Embryo Color     White Percent Mutant Seeds     29.0 % Chi - Square     4.7 Seeds Counted     563 Percent Top Half     46.6 % Chi - Square     0.7 Average Seed Length       Average Embryo Length