SeedGenes Profile: ISPF

Gene Information Gene Symbol ISPF Chromosome Locus At1g63970   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Seed Pigment Predicted Function Isoprenoid Biosynthesis Function Details Plastid MEP pathway Gene Evidence Border Recovery Goodman / Salk (1 allele) Border Recovery Meinke / Riken (1 allele) Database Release December, 2010 Predicted Sequences Gene: 1674 nucleotides mRNA: 956 nucleotides Protein:   231 amino acids cDNA Status FLcDNA Available: BX818620 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (82%) Model Genomes:   Synechocystis (44%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 260324_at

Mutant Information Allele Symbol ispf-1 ispf-2 Mutant Class Seed Pigment Seed Pigment Source of Mutant Myouga / Salk Myouga / Riken Mutant Line Number SALK_002470 RATM12-2658-1 Ecotype Columbia Nossen Genetic Background     ABRC Stock     Mutagen Treatment T-DNA Transposon Insertion Mutant Yes   Details Yes   Details Flanking Sequence     Location of Mutation Exon 1   Terminal Phenotype Pigment Defective Pigment Defective Nomarski Phenotype Allelism Evidence Reference Allele Sequence Identity Database Release December, 2010 December, 2010 Seed Color     Embryo Color     Percent Mutant Seeds 21.6 % 24.3 % Chi - Square 4.1 0.1 Seeds Counted 626 571 Percent Top Half     Chi - Square     Average Seed Length     Average Embryo Length