SeedGenes Profile: KNF

Gene Information Gene Symbol KNF Chromosome Locus At1g67490   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol GCS (L. Lepiniec) Complemetation Test Gene Class Embryo Defective Predicted Function Alpha Glucosidase I Function Details Early step in N-glycan trimming of glycoproteins Gene Evidence Molecular Complementation L. Lepiniec (1 allele) Double Border Recovery L. Lepiniec (2 alleles) Sequenced Mutant Allele C. Somerville (1 allele) Database Release March, 2002 Predicted Sequences Gene: 6146 nucleotides mRNA: 2756 nucleotides Protein:   852 amino acids cDNA Status FLcDNA Available: AK118927 Top BLAST Hits Arabidopsis: AT1G24320.1 (74%) Plant ESTs: Rice (Oryza sativa) (59%) Model Genomes:   Danio rerio (42%) Subcellular Localization TargetP Targeted to Mitochondria SUBA mRNA Expression Data NASCArray Spot History for 264228_at

Mutant Information Allele Symbol knf-14 Mutant Class Embryo Defective Source of Mutant C. Somerville Mutant Line Number   Ecotype Landsberg erecta Genetic Background   ABRC Stock CS6119 Mutagen Treatment EMS Insertion Mutant No Flanking Sequence   Location of Mutation Intron 2 Terminal Phenotype Cotyledon  Details Nomarski Phenotype   Images Allelism Evidence Reference Allele Database Release March, 2002 Seed Color Green Embryo Color Green Percent Mutant Seeds 15.7 % Chi - Square 46.2 Seeds Counted 1005 Percent Top Half 60.1 % Chi - Square 6.5 Average Seed Length 470 ± 50 µm Average Embryo Length 250 ± 50 µm