SeedGenes Profile: KNF

Gene Information Gene Symbol KNF Chromosome Locus At1g67490   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol GCS (L. Lepiniec) Complemetation Test Gene Class Embryo Defective Predicted Function Alpha Glucosidase I Function Details Early step in N-glycan trimming of glycoproteins Gene Evidence Molecular Complementation L. Lepiniec (1 allele) Double Border Recovery L. Lepiniec (2 alleles) Sequenced Mutant Allele C. Somerville (1 allele) Database Release March, 2002 Predicted Sequences Gene: 6146 nucleotides mRNA: 2756 nucleotides Protein:   852 amino acids cDNA Status FLcDNA Available: AK118927 Top BLAST Hits Arabidopsis: AT1G24320.1 (74%) Plant ESTs: Rice (Oryza sativa) (59%) Model Genomes:   Danio rerio (42%) Subcellular Localization TargetP Targeted to Mitochondria SUBA mRNA Expression Data NASCArray Spot History for 264228_at

Mutant Information Allele Symbol knf-14 gcs 1-1 gcs 1-2 Mutant Class Embryo Defective Embryo Defective Embryo Defective Source of Mutant C. Somerville Lepiniec / Versailles Lepiniec / Versailles Mutant Line Number       Ecotype Landsberg erecta Wassilewskija Wassilewskija Genetic Background       ABRC Stock CS6119     Mutagen Treatment EMS T-DNA T-DNA Insertion Mutant No Yes   Details Yes   Details Flanking Sequence       Location of Mutation Intron 2 Intron 8 Intron 15 Terminal Phenotype Cotyledon  Details Cotyledon Cotyledon Nomarski Phenotype   Images Allelism Evidence Reference Allele Sequence Identity Sequence Identity Database Release March, 2002 March, 2002 March, 2002 Seed Color Green     Embryo Color Green     Percent Mutant Seeds 15.7 %     Chi - Square 46.2     Seeds Counted 1005     Percent Top Half 60.1 %     Chi - Square 6.5     Average Seed Length 470 ± 50 µm     Average Embryo Length 250 ± 50 µm