SeedGenes Profile: LEC 1

Gene Information Gene Symbol LEC 1 Chromosome Locus At1g21970   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Transcriptional Regulator Function Details HAP3 subunit of CCAAT-binding transcription factor Gene Evidence Sequenced Mutant Allele J. Harada (1 allele) Molecular Complementation J. Harada (1 allele) Border Recovery J. Harada (1 allele) Database Release March, 2002 Predicted Sequences Gene: 2041 nucleotides mRNA: 936 nucleotides Protein:   238 amino acids cDNA Status FLcDNA Available: BX817959 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (75%) Model Genomes:   None Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 260854_at

Mutant Information Allele Symbol lec 1-2 Mutant Class Embryo Defective Source of Mutant Meinke / Feldmann Mutant Line Number CS2922 Ecotype Wassilewskija Genetic Background   ABRC Stock CS2922 Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence   Location of Mutation Upstream Terminal Phenotype Cotyledon  Details Nomarski Phenotype   Allelism Evidence Complementation Test; Sequence Identity Database Release March, 2002 Seed Color Pale Green Embryo Color Pale Green Percent Mutant Seeds 21.0 % Chi - Square 8.3 Seeds Counted 971 Percent Top Half 43.1 % Chi - Square 3.8 Average Seed Length 510 ± 30 µm Average Embryo Length 420 ± 50 µm