SeedGenes Profile: LEC 1

Gene Information Gene Symbol LEC 1 Chromosome Locus At1g21970   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Transcriptional Regulator Function Details HAP3 subunit of CCAAT-binding transcription factor Gene Evidence Sequenced Mutant Allele J. Harada (1 allele) Molecular Complementation J. Harada (1 allele) Border Recovery J. Harada (1 allele) Database Release March, 2002 Predicted Sequences Gene: 2041 nucleotides mRNA: 936 nucleotides Protein:   238 amino acids cDNA Status FLcDNA Available: BX817959 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (75%) Model Genomes:   None Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 260854_at

Mutant Information Allele Symbol lec 1-1 lec 1-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Feldmann Meinke / Feldmann Mutant Line Number 615 CS2922 Ecotype Wassilewskija Wassilewskija Genetic Background     ABRC Stock CS8101 CS2922 Mutagen Treatment T-DNA T-DNA Insertion Mutant No Yes   Details Flanking Sequence     Location of Mutation Entire Gene Deleted Upstream Terminal Phenotype Cotyledon  Details Cotyledon  Details Nomarski Phenotype Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release March, 2002 March, 2002 Seed Color Pale Green Pale Green Embryo Color Pale Green Pale Green Percent Mutant Seeds 28.7 % 21.0 % Chi - Square 9.1 8.3 Seeds Counted 1218 971 Percent Top Half 50.9 % 43.1 % Chi - Square 0.1 3.8 Average Seed Length 480 ± 30 µm 510 ± 30 µm Average Embryo Length 400 ± 40 µm 420 ± 50 µm