SeedGenes Profile: PDE 166

Gene Information Gene Symbol PDE 166 Chromosome Locus At1g08520   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Seed Pigment Predicted Function Magnesium Chelatase (CHLD) Function Details First committed step in chlorophyll biosynthesis; Insertion of magnesium into protoporphyrin IX Gene Evidence Single Border Recovery Meinke / Syngenta (1 allele) Double Border Recovery Meinke / Syngenta (1 allele) Database Release September, 2002 Predicted Sequences Gene: 4548 nucleotides mRNA: 2549 nucleotides Protein:   760 amino acids cDNA Status FLcDNA Available: AY063821 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (90%) Model Genomes:   Synechocystis (59%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 261695_at

Mutant Information Allele Symbol pde 166-2 Mutant Class Seed Pigment Source of Mutant Meinke / Syngenta Mutant Line Number 13823 Ecotype Columbia Genetic Background   ABRC Stock CS16087 Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence Details  (Help) Location of Mutation Exon 4 Terminal Phenotype Pigment Defective Nomarski Phenotype   Allelism Evidence Complementation Test; Sequence Identity Database Release September, 2002 Seed Color White Embryo Color White Percent Mutant Seeds 28.2 % Chi - Square 5.4 Seeds Counted 100 Percent Top Half 49.8 % Chi - Square <0.1 Average Seed Length   Average Embryo Length