SeedGenes Profile: PDE 166

Gene Information Gene Symbol PDE 166 Chromosome Locus At1g08520   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Seed Pigment Predicted Function Magnesium Chelatase (CHLD) Function Details First committed step in chlorophyll biosynthesis; Insertion of magnesium into protoporphyrin IX Gene Evidence Single Border Recovery Meinke / Syngenta (1 allele) Double Border Recovery Meinke / Syngenta (1 allele) Database Release September, 2002 Predicted Sequences Gene: 4548 nucleotides mRNA: 2549 nucleotides Protein:   760 amino acids cDNA Status FLcDNA Available: AY063821 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (90%) Model Genomes:   Synechocystis (59%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 261695_at

Mutant Information Allele Symbol pde 166-1 pde 166-2 Mutant Class Seed Pigment Seed Pigment Source of Mutant Meinke / Syngenta Meinke / Syngenta Mutant Line Number 11361 13823 Ecotype Columbia Columbia Genetic Background     ABRC Stock CS16086 CS16087 Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help) Details Location of Mutation Exon 1 Exon 4 Terminal Phenotype Pigment Defective Pigment Defective Nomarski Phenotype Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release September, 2002 September, 2002 Seed Color White White Embryo Color White White Percent Mutant Seeds 25.7 % 28.2 % Chi - Square 0.3 5.4 Seeds Counted 983 100 Percent Top Half 51.4 % 49.8 % Chi - Square 0.2 <0.1 Average Seed Length     Average Embryo Length