OSU Syngenta vbi

SeedGenes Profile: PDE 325

Gene Information
Gene Symbol PDE 325
Chromosome Locus At3g51820   tair
Alleles in Database 2   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Seed Pigment
Predicted Function Chlorophyll Synthase (CHLG/ATG4)
Function Details Last step in chlorophyll biosynthesis
Gene Evidence
Border Recovery
Meinke / Salk (2 alleles)
Database Release December, 2010
Predicted Sequences
Gene:3055 nucleotides
mRNA: 1585 nucleotides
Protein:  387 amino acids
cDNA Status FLcDNA Available: AY042821
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Brassica napus (92%)
Model Genomes:  Synechocystis (65%)
Subcellular Localization TargetP Targeted to Chloroplasts
mRNA Expression Data NASCArray Spot History for 246308_at
Mutant Information
Allele Symbol pde 325-1
Mutant Class Seed Pigment
Source of Mutant Meinke / Salk
Mutant Line Number SALK_112733
Ecotype Columbia
Genetic Background  
ABRC Stock  
Mutagen Treatment T-DNA
Insertion Mutant Yes   Details
Flanking Sequence  
Location of Mutation Exon 6
Terminal Phenotype Pigment Defective  Details
Nomarski Phenotype  
Allelism Evidence Reference Allele
Database Release December, 2010
Seed Color White to Pale Yellow
Embryo Color White to Pale Yellow
Percent Mutant Seeds 26.4 %
Chi - Square 0.3
Seeds Counted 254
Percent Top Half 46.3 %
Chi - Square 0.4
Average Seed Length 550 ± 0 µm
Average Embryo Length 450 ± 0 µm