SeedGenes Profile: PDE 325

Gene Information Gene Symbol PDE 325 Chromosome Locus At3g51820   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Seed Pigment Predicted Function Chlorophyll Synthase (CHLG/ATG4) Function Details Last step in chlorophyll biosynthesis Gene Evidence Border Recovery Meinke / Salk (2 alleles) Database Release December, 2010 Predicted Sequences Gene: 3055 nucleotides mRNA: 1585 nucleotides Protein:   387 amino acids cDNA Status FLcDNA Available: AY042821 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (92%) Model Genomes:   Synechocystis (65%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 246308_at

Mutant Information Allele Symbol pde 325-1 pde 325-2 Mutant Class Seed Pigment Seed Pigment Source of Mutant Meinke / Salk Meinke / Salk Mutant Line Number SALK_112733 SALK_134433 Ecotype Columbia Columbia Genetic Background     ABRC Stock     Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence     Location of Mutation Exon 6 Exon 14 Terminal Phenotype Pigment Defective  Details Pigment Defective  Details Nomarski Phenotype Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release December, 2010 December, 2010 Seed Color White to Pale Yellow White to Pale Yellow Embryo Color White to Pale Yellow White to Pale Yellow Percent Mutant Seeds 26.4 % 28.7 % Chi - Square 0.3 1.9 Seeds Counted 254 258 Percent Top Half 46.3 % 50.0 % Chi - Square 0.4 <0.1 Average Seed Length 550 ± 0 µm 550 ± 0 µm Average Embryo Length 450 ± 0 µm 450 ± 0 µm