SeedGenes Profile: PDE 325

Gene Information Gene Symbol PDE 325 Chromosome Locus At3g51820   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Seed Pigment Predicted Function Chlorophyll Synthase (CHLG/ATG4) Function Details Last step in chlorophyll biosynthesis Gene Evidence Border Recovery Meinke / Salk (2 alleles) Database Release December, 2010 Predicted Sequences Gene: 3055 nucleotides mRNA: 1585 nucleotides Protein:   387 amino acids cDNA Status FLcDNA Available: AY042821 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (92%) Model Genomes:   Synechocystis (65%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 246308_at

Mutant Information Allele Symbol pde 325-2 Mutant Class Seed Pigment Source of Mutant Meinke / Salk Mutant Line Number SALK_134433 Ecotype Columbia Genetic Background   ABRC Stock   Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence   Location of Mutation Exon 14 Terminal Phenotype Pigment Defective  Details Nomarski Phenotype   Allelism Evidence Complementation Test; Sequence Identity Database Release December, 2010 Seed Color White to Pale Yellow Embryo Color White to Pale Yellow Percent Mutant Seeds 28.7 % Chi - Square 1.9 Seeds Counted 258 Percent Top Half 50.0 % Chi - Square <0.1 Average Seed Length 550 ± 0 µm Average Embryo Length 450 ± 0 µm