OSU Syngenta vbi

SeedGenes Profile: PDE 325

Gene Information
Gene Symbol PDE 325
Chromosome Locus At3g51820   tair
Alleles in Database 2   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Seed Pigment
Predicted Function Chlorophyll Synthase (CHLG/ATG4)
Function Details Last step in chlorophyll biosynthesis
Gene Evidence
Border Recovery
Meinke / Salk (2 alleles)
Database Release December, 2010
Predicted Sequences
Gene:3055 nucleotides
mRNA: 1585 nucleotides
Protein:  387 amino acids
cDNA Status FLcDNA Available: AY042821
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Brassica napus (92%)
Model Genomes:  Synechocystis (65%)
Subcellular Localization TargetP Targeted to Chloroplasts
mRNA Expression Data NASCArray Spot History for 246308_at
Mutant Information
Allele Symbol pde 325-2
Mutant Class Seed Pigment
Source of Mutant Meinke / Salk
Mutant Line Number SALK_134433
Ecotype Columbia
Genetic Background  
ABRC Stock  
Mutagen Treatment T-DNA
Insertion Mutant Yes   Details
Flanking Sequence  
Location of Mutation Exon 14
Terminal Phenotype Pigment Defective  Details
Nomarski Phenotype  
Allelism Evidence Complementation Test; Sequence Identity
Database Release December, 2010
Seed Color White to Pale Yellow
Embryo Color White to Pale Yellow
Percent Mutant Seeds 28.7 %
Chi - Square 1.9
Seeds Counted 258
Percent Top Half 50.0 %
Chi - Square <0.1
Average Seed Length 550 ± 0 µm
Average Embryo Length 450 ± 0 µm