OSU Syngenta vbi

SeedGenes Profile: PFI

Gene Information
Gene Symbol PFI
Chromosome Locus At1g71440   tair
Alleles in Database 2   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function Tubulin Folding Cofactor E
Function Details Regulation of tubulin folding and microtubule dynamics
Gene Evidence
Sequenced Mutant Allele
U. Mayer (2 alleles)
Database Release September, 2002
Predicted Sequences
Gene:3327 nucleotides
mRNA: 1820 nucleotides
Protein:  531 amino acids
cDNA Status FLcDNA Available: BT002403
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Vitis vinifera (56%)
Model Genomes:  Danio rerio (30%)
Subcellular Localization TargetP Not Targeted to Cell Compartment
SUBA
mRNA Expression Data NASCArray Spot History for 259895_at
    
Mutant Information
Allele Symbol pfi-UU685pfi-UU2221
Mutant Class Embryo DefectiveEmbryo Defective
Source of Mutant U. MayerU. Mayer
Mutant Line Number   
Ecotype Landsberg erectaLandsberg erecta
Genetic Background   
ABRC Stock   
Mutagen Treatment EMSEMS
Insertion Mutant NoNo
Flanking Sequence   
Location of Mutation Exon 1Exon 1
Terminal Phenotype Preglobular  DetailsPreglobular
Nomarski Phenotype Preglobular  Images
Allelism Evidence Reference AlleleComplementation Test; Sequence Identity
Database Release September, 2002September, 2002
Seed Color White to Pale Yellow 
Embryo Color White to Pale Yellow 
Percent Mutant Seeds 41.4 % 
Chi - Square 66.4 
Seeds Counted 461 
Percent Top Half 50.8 % 
Chi - Square <0.1 
Average Seed Length 430 ± 70 µm 
Average Embryo Length 130 ± 80 µm