SeedGenes Profile: RML 1

Gene Information Gene Symbol RML 1 Chromosome Locus At4g23100   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol CAD 2 (C. Cobbett) Sequence Identity Gene Class Embryo Defective Predicted Function Gamma-Glutamylcysteine Synthetase Function Details First enzyme in glutathione biosynthesis; required for initiation and maintenance of cell division Gene Evidence Sequenced Mutant Allele R. Sung (1 allele) Molecular Complementation R. Sung (1 allele) Double Border Recovery Meinke / Syngenta (1 allele) Database Release March, 2002 Predicted Sequences Gene: 4139 nucleotides mRNA: 2055 nucleotides Protein:   522 amino acids cDNA Status FLcDNA Available: AY056372 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (91%) Model Genomes:   None Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 254270_at

Mutant Information Allele Symbol rml 1-1 Mutant Class Embryo Defective Source of Mutant R. Sung Mutant Line Number   Ecotype Columbia Genetic Background   ABRC Stock   Mutagen Treatment EMS Insertion Mutant No Flanking Sequence   Location of Mutation Exon 7 Terminal Phenotype Not Available Nomarski Phenotype   Allelism Evidence Complementation Test; Sequence Identity Database Release March, 2002 Seed Color   Embryo Color   Percent Mutant Seeds   Chi - Square   Seeds Counted   Percent Top Half   Chi - Square   Average Seed Length   Average Embryo Length