OSU Syngenta vbi

SeedGenes Profile: RML 1

Gene Information
Gene Symbol RML 1
Chromosome Locus At4g23100   tair
Alleles in Database 2   See List OR Show All
Identity Confidence Confirmed
Alias Symbol CAD 2 (C. Cobbett) Sequence Identity
Gene Class Embryo Defective
Predicted Function Gamma-Glutamylcysteine Synthetase
Function Details First enzyme in glutathione biosynthesis; required for initiation and maintenance of cell division
Gene Evidence
Sequenced Mutant Allele
R. Sung (1 allele)
Molecular Complementation
R. Sung (1 allele)
Double Border Recovery
Meinke / Syngenta (1 allele)
Database Release March, 2002
Predicted Sequences
Gene:4139 nucleotides
mRNA: 2055 nucleotides
Protein:  522 amino acids
cDNA Status FLcDNA Available: AY056372
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Brassica napus (91%)
Model Genomes:   None
Subcellular Localization TargetP Targeted to Chloroplasts
mRNA Expression Data NASCArray Spot History for 254270_at
Mutant Information
Allele Symbol rml 1-1Srml 1-1
Mutant Class Embryo DefectiveEmbryo Defective
Source of Mutant Meinke / SyngentaR. Sung
Mutant Line Number 23012 
Ecotype ColumbiaColumbia
Genetic Background   
ABRC Stock CS16066 
Mutagen Treatment T-DNAEMS
Insertion Mutant Yes   DetailsNo
Flanking Sequence Details  (Help) 
Location of Mutation Exon 7Exon 7
Terminal Phenotype Cotyledon  DetailsNot Available
Nomarski Phenotype
Allelism Evidence Reference AlleleComplementation Test; Sequence Identity
Database Release March, 2002March, 2002
Seed Color Pale Yellow-Green 
Embryo Color Pale Yellow-Green 
Percent Mutant Seeds 26.2 % 
Chi - Square 0.9 
Seeds Counted 1108 
Percent Top Half 49.1 % 
Chi - Square 0.1 
Average Seed Length 500 ± 40 µm 
Average Embryo Length 390 ± 70 µm