SeedGenes Profile: RML 1

Gene Information Gene Symbol RML 1 Chromosome Locus At4g23100   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol CAD 2 (C. Cobbett) Sequence Identity Gene Class Embryo Defective Predicted Function Gamma-Glutamylcysteine Synthetase Function Details First enzyme in glutathione biosynthesis; required for initiation and maintenance of cell division Gene Evidence Sequenced Mutant Allele R. Sung (1 allele) Molecular Complementation R. Sung (1 allele) Double Border Recovery Meinke / Syngenta (1 allele) Database Release March, 2002 Predicted Sequences Gene: 4139 nucleotides mRNA: 2055 nucleotides Protein:   522 amino acids cDNA Status FLcDNA Available: AY056372 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (91%) Model Genomes:   None Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 254270_at

Mutant Information Allele Symbol rml 1-1S rml 1-1 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta R. Sung Mutant Line Number 23012   Ecotype Columbia Columbia Genetic Background     ABRC Stock CS16066   Mutagen Treatment T-DNA EMS Insertion Mutant Yes   Details No Flanking Sequence Details  (Help)   Location of Mutation Exon 7 Exon 7 Terminal Phenotype Cotyledon  Details Not Available Nomarski Phenotype Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release March, 2002 March, 2002 Seed Color Pale Yellow-Green   Embryo Color Pale Yellow-Green   Percent Mutant Seeds 26.2 %   Chi - Square 0.9   Seeds Counted 1108   Percent Top Half 49.1 %   Chi - Square 0.1   Average Seed Length 500 ± 40 µm   Average Embryo Length 390 ± 70 µm